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基于高效液相色谱外标法测定咔唑含量的研究
Research on the Determination of Carbazole Content Based onHPLC External Standard Method
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- DOI:
- 作者:
- 潘统很，徐炫宗?，戴 雷
Pan Tonghen, Xu Xuanzong?, Dai Lei
- 作者单位:
- 广东阿格蕾雅光电材料有限公司，广东 佛山 528333
Guangdong Aglaia Optoelectronic Materials Co., Ltd., Foshan 528333, China
- 关键词:
- 咔唑；高效液相色谱；外标法；含量
carbazole; HPLC; external standard method; content
- 摘要:
- 摘要：建立基于高效液相色谱外标法测定咔唑含量的方法，以甲醇为溶剂配制标准与样品溶液，色谱条件设置为：流动相A相为甲醇，B相为水，梯度洗脱设置：0~6 min，70%A；6~10 min，70%A~100%A；10~17 min，100%A；后运行时间为 5 min；色谱柱选用艾杰尔 Venusil XBP C184.6 mm×150 mm 5 µm 150A；柱温为 40 ℃；流速为 1.0 mL/min；检测波长为 233 nm；进样量为5 µL。实验结果显示，在 0.5~10 mg/L 浓度范围内，R2为 0.999 9，线性关系良好；试样溶液在48 h内的主峰面积变化、试样6平行检测结果的RSD均小于1.0%，稳定性和精密度良好；检测有值咔唑试样，与标示值的相对平均偏差为0.52%，准确度高；建立了科学可靠的咔唑含量分析方法。
Abstract: A method for determining the content of carbazole based on HPLC external standard method was established, standardand sample solutions were prepared using methanol as the solvent, the chromatographic conditions were set as follows: Mobile phaseA was methanol, Mobile phase B was water, and gradient elution was set: 0-6 min, with 70% A; 6~10 min, 70%A~100%A; 10~17min, 100%A; Post run time: 5 min; Chromatographic column: Agela Venusil XBP C18 4.6 mm×150 mm 5 µm 150A; The columntemperature:40 ℃; Flow rate: 1.0 mL/min; Detection wavelength: 233 nm; Injection volume:5 µL. The experimental results showedthat within the concentration range of 0.5 mg/L to 10 mg/L, the R2 value was 0.999 9, indicating a good linear relationship; The peakarea of carbazole change within 48 h and the results of retest sample for 6 times,the RSD both less than 1.0%, indicating goodstability and precision; detection the known content carbazole samples showed a relative average deviation of 0.52% from thelabeled values, indicating high accuracy; Conclusion: A scientifically reliable method for analyzing the content of carbazole has beenestablished.