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氧化石墨烯生物电极的构筑与性能研究
Research on the Construction and Performance ofGraphene Oxide Bioelectrodes
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- DOI:
- 作者:
- 田 娟
Tian Juan
- 作者单位:
- （朔州职业技术学院，山西 朔州 036000）
(Shuozhou Vocational and Technical College, Shuozhou 036000, China)
- 关键词:
- 氧化石墨烯；生物电极；葡萄糖氧化酶
graphene oxide; bioelectrode; glucose oxidase
- 摘要:
- 摘要：利用层层组装的方法通过双功能基团的1-芘丁酸N-羟基琥珀酰亚胺酯（PANHS），把葡萄糖氧化酶（GOD）和氧化石墨烯（GO）链接到Pt电极表面，制备了高稳定性的Pt/GO/PANHS/GOD 生物传感系统。在 0.6 V 操作电位下，该体系对葡萄糖有较宽的线性响应范围（200×10-4~42×10-4 M），快速的响应时间（小于 10 s），高灵敏度（21.8 mA/（cm2∙M））以及低的检测限（3 µM，S/N=3）。利用扫描隧道电子显微镜（SEM）表征了组装过程，结果表明，GO薄膜的“褶皱结构”可以为PANHS的键合提供有利的环境，而PANHS的引入极大地增加了GOD的固载量，使得Pt/GO/PANHS/GOD传感器对葡萄糖表现出了明显的电催化氧化活性。
Abstract: A highly stable biological film was formed on the functional platinum plate electrode via layer-by-layer assembly of 1-pyrenebutanoie acid and succinimidyl ester (PANHS), GOD, Graphene oxide (GO). The bioelectrode showed electrocatalytic activitytoward the oxidation of glucose and it was successfully applied to the determination of glucose in amperometric mode at 0.6 V (vs.SCE). The biosensor exhibited excellent performance during glucose determination with high sensitivity (21.8 mA/(cm2∙M)), a widelinear range (2.0 µM~4.2 mM), a fast response time (10 s)and a low detection limit (3 µM, S / N=3). Furthermore, the biosensorexhibited excellent long-term stability, and satisfactory reproducibility.